Search results for "Calcium dynamics"

showing 2 items of 2 documents

Photocatalytic Activity of Polymer Nanoparticles Modulates Intracellular Calcium Dynamics and Reactive Oxygen Species in HEK-293 Cells

2018

Optical modulation of living cells activity by light-absorbing exogenous materials is gaining increasing interest, due to the possibility both to achieve high spatial and temporal resolution with a minimally invasive and reversible technique and to avoid the need of viral transfection with light-sensitive proteins. In this context, conjugated polymers represent ideal candidates for photo-transduction, due to their excellent optoelectronic and biocompatibility properties. In this work, we demonstrate that organic polymer nanoparticles, based on poly(3-hexylthiophene) conjugated polymer, establish a functional interaction with an in vitro cell model (Human Embryonic Kidney cells, HEK-293). Th…

0301 basic medicineconjugated polymerHistologylcsh:BiotechnologyCellBiomedical EngineeringBioengineeringContext (language use)02 engineering and technologyCalcium in biology03 medical and health sciencesCa2+ imaginglcsh:TP248.13-248.65medicineViability assaybio-organic electronicsOriginal Researchreactive oxygen specieschemistry.chemical_classificationReactive oxygen speciesorganic semiconductorHEK 293 cellsBioengineering and BiotechnologyPhotocatalytic ActivityTransfection021001 nanoscience & nanotechnologyConjugated Polymer NanoparticlesCytosol030104 developmental biologymedicine.anatomical_structurechemistryBiophysicsIntracellular Calcium Dynamicsphotomodulationlight0210 nano-technologycell optical stimulationBiotechnologyFrontiers in Bioengineering and Biotechnology
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Simulations of the cultured granule neuron excitability

2003

Abstract We have developed a biophysical model of a cultured rat cerebellar granule neuron and simulated its excitability under different experimental conditions. The basic excitability properties of such a small neuron; the specific action potential waveforms, the overall firing patterns induced by current stimulations, and the linear frequency-current relation, are the main model constraints. Simulations show that for a one-compartmental granule neuron model, the constraints are met using six voltage- and time-dependent ion channel types and calcium dynamics linked to BK Ca ion channel function. This kind of model of a single neuron forms a solid basis for building the increasingly more c…

CerebellumQuantitative Biology::Neurons and CognitionChemistryCognitive NeuroscienceBiological neuron modelSmall neuronComputer Science Applicationsmedicine.anatomical_structureGranular cellnervous systemArtificial IntelligenceCalcium dynamicsmedicineBiological neural networkNeuronNeuroscienceIon channelNeurocomputing
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